B4GALNT3 Antibody

ProSci
Product Code: PSI-57-182
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-182-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in 293 cell line lysates (35ug/lane).
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B4GALNT3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in 293 cell line lysates (35ug/lane).
B4GALNT3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Beta-1,4-N-acetylgalactosaminyltransferase 3, B4GalNAcT3, Beta4GalNAc-T3, Beta4GalNAcT3, Beta-1,4-N-acetylgalactosaminyltransferase III, N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 2, NGalNAc-T2, B4GALNT3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
B4GALNT3 transfers N-acetylgalactosamine (GalNAc) onto glucosyl residues to form N,N-prime-diacetyllactosediamine (LacdiNAc, or LDN), a unique terminal structure of cell surface N-glycans (Ikehara et al., 2006 [PubMed 16728562]).[supplied by OMIM].
Background References:
  • Rose, J. Phd, et al. Mol. Med. (2010) In press :
  • Arking, D.E., et al. PLoS ONE(3), E9879 (2010) :
  • Miller, E., et al. J. Biol. Chem. 283(4):1985-1991(2008)
  • Huang, J., et al. Mol. Cancer Res. 5(6):543-552(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This B4GALNT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 555-584 amino acids from the Central region of human B4GALNT3.
NCBI Gene ID #:
283358
NCBI Official Name:
Beta-1,4-N-acetylgalactosaminyltransferase 3
NCBI Official Symbol:
B4GALNT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
115 kDa
Protein Accession #:
Q6L9W6
Protein GI Number:
161789024
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q6L9W6
User NOte:
Optimal dilutions for each application to be determined by the researcher.