FARSB Antibody

ProSci
Product Code: PSI-57-469
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-469-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis in Y79 cell line lysates (35ug/lane).
2 / 2
FARSB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in Y79 cell line lysates (35ug/lane).
FARSB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Phenylalanine--tRNA ligase beta subunit, Phenylalanyl-tRNA synthetase beta subunit, PheRS, FARSB, FARSLB, FRSB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Finarov, I., et al. Structure 18(3):343-353(2010)
  • Yu, X.Y., et al. Bioorg. Med. Chem. Lett. 14(5):1339-1342(2004)
  • Vasil'eva, I.A., et al. Biochemistry Mosc. 69(2):143-153(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FARSB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-564 amino acids from the C-terminal region of human FARSB.
NCBI Gene ID #:
10056
NCBI Official Name:
Phenylalanine--tRNA ligase beta subunit
NCBI Official Symbol:
FARSB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
Q9NSD9
Protein GI Number:
296452943
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9NSD9
User NOte:
Optimal dilutions for each application to be determined by the researcher.