ZNF175 Antibody

ProSci
Product Code: PSI-61-044
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-044-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H.heart section using ZNF175 Antibody (N-term). Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
2 / 2
Western blot analysis of lysates from A431, Jurkat, K562, MDA-MB-231, U-251 MG cell line (from left to right), using ZNF175 Antibody at 1:1000 at each lane.

Immunohistochemical analysis of paraffin-embedded H.heart section using ZNF175 Antibody (N-term). Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from A431, Jurkat, K562, MDA-MB-231, U-251 MG cell line (from left to right), using ZNF175 Antibody at 1:1000 at each lane.

Further Information

Additional Names:
Zinc finger protein 175, Zinc finger protein OTK18, ZNF175
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Down-regulates the expression of several chemokine receptors. Interferes with HIV-1 replication by suppressing Tat- induced viral LTR promoter activity.
Background References:
  • Saito H.,et al.Genomics 31:376-379(1996).
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Grimwood J.,et al.Nature 428:529-535(2004).
  • Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ZNF175 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 150-187 amino acids from the N-terminal region of human ZNF175.
NCBI Gene ID #:
7728
NCBI Official Name:
Zinc finger protein 175
NCBI Official Symbol:
ZNF175
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
82 kDa
Protein Accession #:
Q9Y473
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9Y473
User NOte:
Optimal dilutions for each application to be determined by the researcher.