DEGS2 Antibody

ProSci
Product Code: PSI-55-272
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-272-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis in Hela cell line lysates (35ug/lane).
2 / 2
DEGS2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in Hela cell line lysates (35ug/lane).
DEGS2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Sphingolipid delta(4)-desaturase/C4-hydroxylase DES2, 1---, 114--, Degenerative spermatocyte homolog 2, DEGS2 (HGNC:20113), C14orf66
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
DEGS2 encodes a bifunctional enzyme that is involved in the biosynthesis of phytosphingolipids in human skin and in other phytosphingolipid-containing tissues. This enzyme can act as a sphingolipid delta(4)-desaturase, and also as a sphingolipid C4-hydroxylase.
Background References:
  • Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
  • Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
  • Mizutani, Y., et al. FEBS Lett. 563 (1-3), 93-97 (2004) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This DEGS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-290 amino acids from the C-terminal region of human DEGS2.
NCBI Gene ID #:
123099
NCBI Official Name:
Sphingolipid delta(4)-desaturase/C4-hydroxylase DES2
NCBI Official Symbol:
DEGS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
Q6QHC5
Protein GI Number:
269849561
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q6QHC5
User NOte:
Optimal dilutions for each application to be determined by the researcher.