CSRP2 Antibody

ProSci
Product Code: PSI-57-459
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-459-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
2 / 3
Western blot analysis of CSRP2 using rabbit polyclonal CSRP2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CSRP2 gene.
3 / 3

Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Western blot analysis of CSRP2 using rabbit polyclonal CSRP2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CSRP2 gene.

Further Information

Additional Names:
Cysteine and glycine-rich protein 2, Cysteine-rich protein 2, CRP2, LIM domain only protein 5, LMO-5, Smooth muscle cell LIM protein, SmLIM, CSRP2, LMO5, SMLIM
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
CSRP2 is a member of the CSRP family of genes, encoding a group of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. CRP2 contains two copies of the cysteine-rich amino acid sequence motif (LIM) with putative zinc-binding activity, and may be involved in regulating ordered cell growth. Other genes in the family include CSRP1 and CSRP3.
Background References:
  • Stearns, M.E., et al. Mol. Cancer Res. 1(9):631-642(2003)
  • Wang, M., et al. Cancer Biol. Ther. 1(5):556-563(2002)
  • Weiskirchen, R., et al. Biochem. J. 359 (PT 3), 485-496 (2001) :
  • Weiskirchen, R., et al. Biochem. Biophys. Res. Commun. 274(3):655-663(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This CSRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the Central region of human CSRP2.
NCBI Gene ID #:
1466
NCBI Official Name:
Cysteine and glycine-rich protein 2
NCBI Official Symbol:
CSRP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
Q16527
Protein GI Number:
2497674
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q16527
User NOte:
Optimal dilutions for each application to be determined by the researcher.