E2EPF Antibody

ProSci
Product Code: PSI-61-083
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-083-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
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E2EPF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
E2EPF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Ubiquitin-conjugating enzyme E2 S, E2-EPF, Ubiquitin carrier protein S, Ubiquitin-conjugating enzyme E2-24 kDa, Ubiquitin-conjugating enzyme E2-EPF5, Ubiquitin-protein ligase S, UBE2S, E2EPF
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.
Background References:
  • Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
  • Liu, Z., et al., J. Biol. Chem. 267(22):15829-15835 (1992).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine
Immunogen:
This E2EPF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-222 amino acids from the C-terminal region of human E2EPF.
NCBI Gene ID #:
27338
NCBI Official Name:
Ubiquitin-conjugating enzyme E2 S
NCBI Official Symbol:
UBE2S
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
Q16763
Protein GI Number:
23829978
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q16763
User NOte:
Optimal dilutions for each application to be determined by the researcher.