DUT Antibody

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ProSci
Product Code: PSI-57-557
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-557-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + SW620 whole cell lysates Lysates/proteins at 20 ug per lane.
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Western blot analysis of lysate from A431 cell line, using DUT Antibody at 1:1000 at each lane.
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Western Blot at 1:1000 dilution + SW620 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of lysate from A431 cell line, using DUT Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, dUTPase, dUTP pyrophosphatase, DUT
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Background References:
  • Takatori, H., et al. Liver Int. 30(3):438-446(2010) Quesada-Soriano, I., et al. Biochimie 92(2):178-186(2010) Chanson, A., et al. Am. J. Clin. Nutr. 89(6):1927-1936(2009) Takacs, E., et al. FEBS Lett. 583(5):865-871(2009) Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This DUT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the C-terminal region of human DUT.
NCBI Gene ID #:
1854
NCBI Official Name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
NCBI Official Symbol:
DUT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
P33316
Protein GI Number:
347595814
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P33316
User NOte:
Optimal dilutions for each application to be determined by the researcher.