Dnmt3a Antibody

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ProSci
Product Code: PSI-55-137
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-137-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-Dnmt3a Pab in T47-D cell lysate
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Lysates from mice thymus tissue after radiation were subjected to WB using antibody against DNMT3a. CT, control animals; FR, animals subjected to fractionated exposure; AC, acutely exposed animals. All sample loading was normalized to protein content. Representative Western blots from three independent experiments are shown; each lane represents a protein extract of a thymus of one animal. (Mol. Cancer Res. 2005 Oct 01;3(10):553-561)
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Western blot analysis of anti-Dnmt3a Pab in T47-D cell lysate
Lysates from mice thymus tissue after radiation were subjected to WB using antibody against DNMT3a. CT, control animals; FR, animals subjected to fractionated exposure; AC, acutely exposed animals. All sample loading was normalized to protein content. Representative Western blots from three independent experiments are shown; each lane represents a protein extract of a thymus of one animal. (Mol. Cancer Res. 2005 Oct 01;3(10):553-561)

Documents

Further Information

Additional Names:
DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Background References:
  • Xie, S., et al., Gene 236(1):87-95 (1999). Robertson, K.D., et al., Nucleic Acids Res. 27(11):2291-2298 (1999).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Chicken
Immunogen:
This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from human Dnmt3a.
NCBI Gene ID #:
1788
NCBI Official Name:
DNA (cytosine-5)-methyltransferase 3A
NCBI Official Symbol:
DNMT3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
102 kDa
Protein Accession #:
Q9Y6K1
Protein GI Number:
166215081
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q9Y6K1
User NOte:
Optimal dilutions for each application to be determined by the researcher.