PP5 Antibody

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ProSci
Product Code: PSI-63-489
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-489-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Serine/threonine-protein phosphatase 5, PP5, Protein phosphatase T, PP-T, PPT, PPP5C, PPP5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
PPP5C belongs to the PPP phosphatase family, PP-T subfamily, and may play a role in the regulation of RNA biogenesis and/or mitosis. In vitro,PPP5C dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
Background References:
  • Yang, J., et al., EMBO J. 24(1):1-10 (2005).
  • Zhou, G., et al., J. Biol. Chem. 279(45):46595-46605 (2004).
  • Huang, S., et al., J. Biol. Chem. 279(35):36490-36496 (2004).
  • Swingle, M.R., et al., J. Biol. Chem. 279(32):33992-33999 (2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This PP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-475 amino acids from the C-terminal region of human PP5 .
NCBI Gene ID #:
5536
NCBI Official Name:
Serine/threonine-protein phosphatase 5
NCBI Official Symbol:
PPP5C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
P53041
Protein GI Number:
1709744
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P53041
User NOte:
Optimal dilutions for each application to be determined by the researcher.