MRPS12 Antibody
| Code | Size | Price |
|---|
| PSI-56-556-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
28S ribosomal protein S12, mitochondrial, MRP-S12, S12mt, MT-RPS12, MRPS12, RPMS12, RPSM12
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
Mammalian mitochondrial ribosomal proteins are encoded by
nuclear genes and help in protein synthesis within the
mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
small 28S subunit and a large 39S subunit. They have an estimated
75% protein to rRNA composition compared to prokaryotic ribosomes,
where this ratio is reversed. Another difference between mammalian
mitoribosomes and prokaryotic ribosomes is that the latter contain
a 5S rRNA. Among different species, the proteins comprising the
mitoribosome differ greatly in sequence, and sometimes in
biochemical properties, which prevents easy recognition by sequence
homology. This gene encodes a 28S subunit protein that belongs to
the ribosomal protein S12P family. The encoded protein is a key
component of the ribosomal small subunit and controls the decoding
fidelity and susceptibility to aminoglycoside antibiotics. The gene
for mitochondrial seryl-tRNA synthetase is located upstream and
adjacent to this gene, and both genes are possible candidates for
the autosomal dominant deafness gene (DFNA4). Splice variants that
differ in the 5' UTR have been found for this gene; all three
variants encode the same protein.
Background References:
- Zanotto, E., et al. Biochim. Biophys. Acta 1789(5):432-442(2009)
- Russo, A., et al. Biochim. Biophys. Acta 1779(12):820-829(2008)
- Stelzl, U., et al. Cell 122(6):957-968(2005)
- Cui, Y.P., et al. World J. Gastroenterol. 9(9):1892-1896(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MRPS12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-59 amino acids from the Central region of human MRPS12.
NCBI Gene ID #:
6183
NCBI Official Name:
28S ribosomal protein S12, mitochondrial
NCBI Official Symbol:
MRPS12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
15 kDa
Protein Accession #:
O15235
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O15235
User NOte:
Optimal dilutions for each application to be determined by the researcher.