EIF4B Antibody

Contact us
ProSci
Product Code: PSI-60-584
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-584-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis of anti-EIF4B Antibody Pab in HepG2 cell line lysates (35ug/lane).
3 / 4
4 / 4
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
❮❯

Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-EIF4B Antibody Pab in HepG2 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-382 amino acids from the Central region of human EIF4B.
NCBI Gene ID #:
1975
NCBI Official Name:
Eukaryotic translation initiation factor 4B
NCBI Official Symbol:
EIF4B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
69 kDa
Protein Accession #:
P23588
Protein GI Number:
205371761
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P23588
User NOte:
Optimal dilutions for each application to be determined by the researcher.