DDX27 Antibody

ProSci
Product Code: PSI-55-630
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-630-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
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DDX27 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prsotate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in K562 cell line lysates (35ug/lane).
DDX27 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prsotate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Probable ATP-dependent RNA helicase DDX27, DEAD box protein 27, DDX27, RHLP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined. [provided by RefSeq].
Background References:
  • Deloukas, P., et al. Nature 414(6866):865-871(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This DDX27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 770-796 amino acids from the C-terminal region of human DDX27.
NCBI Gene ID #:
55661
NCBI Official Name:
Probable ATP-dependent RNA helicase DDX27
NCBI Official Symbol:
DDX27
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
90 kDa
Protein Accession #:
Q96GQ7
Protein GI Number:
29427946
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96GQ7
User NOte:
Optimal dilutions for each application to be determined by the researcher.