RARS2 Antibody

ProSci
Product Code: PSI-63-535
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-535-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of RARS2 Antibody in HepG2 cell line lysates (35ug/lane)
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Flow cytometric analysis of NCI-H292 cells using RARS2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of RARS2 Antibody in HepG2 cell line lysates (35ug/lane)
Flow cytometric analysis of NCI-H292 cells using RARS2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Probable arginine--tRNA ligase, mitochondrial, Arginyl-tRNA synthetase, ArgRS, RARS2, RARSL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
RARS2 is an arginyl-tRNA synthetase that is found in the mitochondrial matrix.
Background References:
  • Edvardson,S., et.al., Am. J. Hum. Genet. 81 (4), 857-862 (2007)
  • Ewing,R.M., et.al., Mol. Syst. Biol. 3, 89 (2007)
  • Bonnefond,L., et.al., Biochemistry 44 (12), 4805-4816 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This RARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-399 amino acids from the C-terminal region of human RARS2.
NCBI Gene ID #:
57038
NCBI Official Name:
Probable arginine--tRNA ligase, mitochondrial
NCBI Official Symbol:
RARS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
Q5T160
Protein GI Number:
74744409
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q5T160
User NOte:
Optimal dilutions for each application to be determined by the researcher.