RPL31 Antibody

ProSci
Product Code: PSI-63-993
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-993-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of RPL31 Antibody in Hela cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human testis tissue reacted with RPL31 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western blot analysis of RPL31 Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with RPL31 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
60S ribosomal protein L31, RPL31
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL31 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L31E family of ribosomal proteins. It is located in the cytoplasm.
Background References:
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Hillier, L.W., et al. Nature 434(7034):724-731(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat, Xenopus
Immunogen:
This RPL31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the Central region of human RPL31.
NCBI Gene ID #:
6160
NCBI Official Name:
60S ribosomal protein L31
NCBI Official Symbol:
RPL31
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
14 kDa
Protein Accession #:
P62899
Protein GI Number:
51702807
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P62899
User NOte:
Optimal dilutions for each application to be determined by the researcher.