ACF Antibody
| Code | Size | Price |
|---|
| PSI-55-326-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
APOBEC1 complementation factor, APOBEC1-stimulating protein, A1CF, ACF, ASP {ECO:0000312|EMBL:CAB947541}
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Background:
Mammalian apolipoprotein B mRNA undergoes site-specific C
to U deamination, which is mediated by a multi-component enzyme
complex containing a minimal core composed of APOBEC-1 and a
complementation factor encoded by this gene. The gene product has
three non-identical RNA recognition motifs and belongs to the hnRNP
R family of RNA-binding proteins. It has been proposed that this
complementation factor functions as an RNA-binding subunit and
docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest
that the protein may also be involved in other RNA editing or RNA
processing events.
Background References:
- Galloway, C.A., et al. Biochem. Biophys. Res. Commun. 391(1):659-663(2010)
- Blanc, V., et al. Mol. Cell. Biol. 25(16):7260-7269(2005)
- Deloukas, P., et al. Nature 429(6990):375-381(2004)
- Xie, K., et al. Proc. Natl. Acad. Sci. U.S.A. 101(21):8114-8119(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human ACF.
NCBI Gene ID #:
29974
NCBI Official Name:
APOBEC1 complementation factor
NCBI Official Symbol:
A1CF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
Q9NQ94
Protein GI Number:
74761651
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9NQ94
User NOte:
Optimal dilutions for each application to be determined by the researcher.