SGF29 Antibody

ProSci
Product Code: PSI-61-809
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-809-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis of SGF29 Antibody in MDA-MB435 cell line lysates (35ug/lane)
2 / 3
SGF29 Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western blot analysis of SGF29 Antibody in MDA-MB435 cell line lysates (35ug/lane)
SGF29 Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
SAGA-associated factor 29 homolog, Coiled-coil domain-containing protein 101, CCDC101, SGF29
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
CCDC101 is a subunit of 2 histone acetyltransferase complexes: the ADA2A (TADA2A; MIM 602276)-containing (ATAC) complex and the SPT3 (SUPT3H; MIM 602947)-TAF9 (MIM 600822)-GCN5 (KAT2A; MIM 602301)/PCAF (KAT2B; MIM 602303) acetylase (STAGA) complex. Both of these complexes contain either GCN5 or PCAF, which are paralogous acetyltransferases.
Background References:
  • Imielinski, M., et al. Nat. Genet. 41(12):1335-1340(2009)
  • Wang, Y.L., et al. J. Biol. Chem. 283(49):33808-33815(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat
Immunogen:
This SGF29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-208 amino acids from the C-terminal region of human SGF29.
NCBI Gene ID #:
112869
NCBI Official Name:
SAGA-associated factor 29 homolog
NCBI Official Symbol:
CCDC101
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
Q96ES7
Protein GI Number:
74731608
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96ES7
User NOte:
Optimal dilutions for each application to be determined by the researcher.