E2F5 Antibody

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ProSci
Product Code: PSI-63-739
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-739-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with E2F5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with E2F5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Transcription factor E2F5, E2F-5, E2F5
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.
Background References:
  • Itoh,A., et.al., Cell. Mol. Biol. Res. 41 (3), 147-154 (1995)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Xenopus
Immunogen:
This E2F5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the N-terminal region of human E2F5.
NCBI Gene ID #:
1875
NCBI Official Name:
Transcription factor E2F5
NCBI Official Symbol:
E2F5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
Q15329
Protein GI Number:
2494230
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q15329
User NOte:
Optimal dilutions for each application to be determined by the researcher.