GALNT3 Antibody

ProSci
Product Code: PSI-63-838
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-838-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of GALNT3 Antibody in MCF-7 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human skin tissue reacted with GALNT3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of GALNT3 Antibody in MCF-7 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skin tissue reacted with GALNT3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide GalNAc transferase 3, GalNAc-T3, pp-GaNTase 3, Protein-UDP acetylgalactosaminyltransferase 3, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3, GALNT3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
GALNT3 encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein is highly homologous to other family members, however the enzymes have different substrate specificities.
Background References:
  • Joseph,L., et.al., Skeletal Radiol. 39 (1), 63-68 (2010)
  • Masi,L.,et.al.,Bone Joint Surg Am 91 (5), 1190-1198 (2009)
  • Chefetz,I., et.al, Biochim. Biophys. Acta 1792 (1), 61-67 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GALNT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 454-481 amino acids from the Central region of human GALNT3.
NCBI Gene ID #:
2591
NCBI Official Name:
Polypeptide N-acetylgalactosaminyltransferase 3
NCBI Official Symbol:
GALNT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
73 kDa
Protein Accession #:
Q14435
Protein GI Number:
209572629
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q14435
User NOte:
Optimal dilutions for each application to be determined by the researcher.