TFAP4 Antibody

ProSci
Product Code: PSI-61-931
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-931-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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TFAP4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
TFAP4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Transcription factor AP-4, Activating enhancer-binding protein 4, Class C basic helix-loop-helix protein 41, bHLHc41, TFAP4, BHLHC41
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization.
Background References:
  • Ku, W.C., et al. Mol. Cell Proteomics 8(9):2034-2050(2009)
  • Jung, P., et al. Proc. Natl. Acad. Sci. U.S.A. 105(39):15046-15051(2008)
  • Pons, J., et al. Biochemistry 47(1):14-29(2008)
  • Mermod, N., et al. Nature 332(6164):557-561(1988)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TFAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-334 amino acids from the C-terminal region of human TFAP4.
NCBI Gene ID #:
7023
NCBI Official Name:
Transcription factor AP-4
NCBI Official Symbol:
TFAP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
Q01664
Protein GI Number:
1729833
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q01664
User NOte:
Optimal dilutions for each application to be determined by the researcher.