MRPS24 Antibody

ProSci
Product Code: PSI-55-220
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-220-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A2058 cell line lysates (35ug/lane).
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MRPS24 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in A2058 cell line lysates (35ug/lane).
MRPS24 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
28S ribosomal protein S24, mitochondrial, MRP-S24, S24mt, bMRP-47, bMRP47, MRPS24
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS24 encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 11. [provided by RefSeq].
Background References:
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
  • Zhang, Z., et al. Genomics 81(5):468-480(2003)
  • Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MRPS24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the Central region of human MRPS24.
NCBI Gene ID #:
64951
NCBI Official Name:
28S ribosomal protein S24, mitochondrial
NCBI Official Symbol:
MRPS24
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
Q96EL2
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96EL2
User NOte:
Optimal dilutions for each application to be determined by the researcher.