RPS3A Antibody

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ProSci
Product Code: PSI-61-964
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-964-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Hela,A2058 cell line lysates (15ug/lane).
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RPS3A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in Hela,A2058 cell line lysates (15ug/lane).
RPS3A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
40S ribosomal protein S3a {ECO:0000255|HAMAP-Rule:MF_03122}, v-fos transformation effector protein, Fte-1, RPS3A {ECO:0000255|HAMAP-Rule:MF_03122}, FTE1, MFTL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Background References:
  • Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Jonson, L., et al. Mol. Cell Proteomics 6(5):798-811(2007)
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Monkey, Mouse, Rat
Immunogen:
This RPS3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-254 amino acids from the C-terminal region of human RPS3A.
NCBI Gene ID #:
6189
NCBI Official Name:
40S ribosomal protein S3a {ECO:0000255|HAMAP-Rule:MF_03122}
NCBI Official Symbol:
RPS3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
P61247
Protein GI Number:
47117764
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P61247
User NOte:
Optimal dilutions for each application to be determined by the researcher.