RPS13 Antibody

ProSci
Product Code: PSI-61-671
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-671-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of RPS13 Antibody in NCI-H460 cell line lysates (35ug/lane)
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RPS13 Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of RPS13 Antibody in NCI-H460 cell line lysates (35ug/lane)
RPS13 Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
40S ribosomal protein S13, RPS13
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
RPS13, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Background References:
  • Malygin, A.A., et al. Nucleic Acids Res. 35(19):6414-6423(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Yu, Y., et al. Protein Sci. 14(6):1438-1446(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Hamster, Mouse, Rat, Xenopus
Immunogen:
This RPS13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the Central region of human RPS13.
NCBI Gene ID #:
6207
NCBI Official Name:
40S ribosomal protein S13
NCBI Official Symbol:
RPS13
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
P62277
Protein GI Number:
50403608
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P62277
User NOte:
Optimal dilutions for each application to be determined by the researcher.