U2AF1 Antibody

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ProSci
Product Code: PSI-61-517
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-517-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti- in Ramos cell line lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of U2AF1 using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.
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Western blot analysis of anti- in Ramos cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of U2AF1 using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.

Documents

Further Information

Additional Names:
Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.
Background References:
  • Rino,J., Mol. Cell. Biol. 28 (9), 3045-3057 (2008) Little,J.T., J. Biol. Chem. 283 (13), 8145-8152 (2008) Pacheco,T.R., Mol. Cell. Biol. 26 (21), 8183-8190 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1.
NCBI Gene ID #:
102724594, 7307
NCBI Official Name:
Splicing factor U2AF 35 kDa subunit
NCBI Official Symbol:
U2AF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
Q01081
Protein GI Number:
267187
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q01081
User NOte:
Optimal dilutions for each application to be determined by the researcher.