EIF2C2 Antibody

ProSci
Product Code: PSI-60-283
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-283-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF2C2 antibody detected the EIF2C2 protein (arrow).
2 / 3
EIF2C2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF2C2 antibody detected the EIF2C2 protein (arrow).
EIF2C2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Protein argonaute-2 {ECO:0000255|HAMAP-Rule:MF_03031}, Argonaute2 {ECO:0000255|HAMAP-Rule:MF_03031}, mAgo2, 3126n2 {ECO:0000255|HAMAP-Rule:MF_03031}, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}, eIF-2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}, eIF2C 2 {ECO:0000255|HAMAP-Rule:MF_03031}, Piwi/argonaute family protein meIF2C2, Protein slicer {ECO:0000255|HAMAP-Rule:MF_03031}, Ago2, Eif2c2, Kiaa4215
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
EIF2C2 is a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.
Background References:
  • Rivas, F.V., et al., Nat Struct Mol Biol 12(4):340-349 (2005).
  • Liu, J., et al., Science 305(5689):1437-1441 (2004).
  • Nelson, P.T., et al., RNA 10(3):387-394 (2004).
  • Sasaki, T., et al., Genomics 82(3):323-330 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rabbit, Rat
Immunogen:
This EIF2C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-59 amino acids from the N-terminal region of human EIF2C2.
NCBI Gene ID #:
239528
NCBI Official Name:
Protein argonaute-2 {ECO:0000255|HAMAP-Rule:MF_03031}
NCBI Official Symbol:
Ago2
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Protein Accession #:
Q8CJG0
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8CJG0
User NOte:
Optimal dilutions for each application to be determined by the researcher.