ND2 Antibody

ProSci
Product Code: PSI-63-922
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-922-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of ND2 Antibody in K562 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with ND2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of ND2 Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ND2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
NADH-ubiquinone oxidoreductase chain 2, NADH dehydrogenase subunit 2, MT-ND2, MTND2, NADH2, ND2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ND2 is core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. This protein complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
Background References:
  • Saitoh,K., et.al., J. Mol. Evol. 63 (6), 826-841 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ND2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-59 amino acids from the N-terminal region of human ND2.
NCBI Gene ID #:
4536
NCBI Official Name:
NADH-ubiquinone oxidoreductase chain 2
NCBI Official Symbol:
MT-ND2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
P03891
Protein GI Number:
128676
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P03891
User NOte:
Optimal dilutions for each application to be determined by the researcher.