HBG2 Antibody

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ProSci
Product Code: PSI-61-057
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-057-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. liver section using HBG2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Western blot analysis of lysates from K562 cell line and human placenta tissue lysate (from left to right), using HBG2 Antibody . was diluted at 1:1000 at each lane.
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Immunohistochemical analysis of paraffin-embedded H. liver section using HBG2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from K562 cell line and human placenta tissue lysate (from left to right), using HBG2 Antibody . was diluted at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Hemoglobin subunit gamma-2, Gamma-2-globin, Hb F Ggamma, Hemoglobin gamma-2 chain, Hemoglobin gamma-G chain, HBG2
Application Note:
For IHC-P starting dilution is: 1:25

For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.
Background References:
  • Slightom J.L.,et al.Cell 21:627-638(1980).
  • Cavallesco C.,et al.Gene 12:215-221(1980).
  • Kutlar F.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HBG2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 101-133 amino acids from the C-terminal region of human HBG2.
NCBI Gene ID #:
3048
NCBI Official Name:
Hemoglobin subunit gamma-2
NCBI Official Symbol:
HBG2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
16 kDa
Protein Accession #:
P69892
Protein GI Number:
56749861
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P69892
User NOte:
Optimal dilutions for each application to be determined by the researcher.