RFC2 Antibody

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ProSci
Product Code: PSI-61-510
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-510-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
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Rat lung, taken with 40x objective. Immunohistochemistry with RFC2 Antibody (N-term), 1:200 dilution,counter stained with Hematoxylin. Positive cells identified with arrows. Av-Alveoli, Br-Bronchus, Pa-Pulmonary Artery. (Provided by Hirotaka Ata,University of South Alabama, Dept of Biochem and Mol Biol)
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Western Blot at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Rat lung, taken with 40x objective. Immunohistochemistry with RFC2 Antibody (N-term), 1:200 dilution,counter stained with Hematoxylin. Positive cells identified with arrows. Av-Alveoli, Br-Bronchus, Pa-Pulmonary Artery. (Provided by Hirotaka Ata,University of South Alabama, Dept of Biochem and Mol Biol)

Documents

Further Information

Additional Names:
Replication factor C subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, Activator 1 subunit 2, Replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40, RFC2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. RFC2 is the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of RFC2 gene has been associated with Williams syndrome.
Background References:
  • Tomida,J., J. Biol. Chem. 283 (14), 9071-9079 (2008) Gupte,R.S., Cell Cycle(2), 323-329 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This RFC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human RFC2.
NCBI Gene ID #:
5982
NCBI Official Name:
Replication factor C subunit 2
NCBI Official Symbol:
RFC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
P35250
Protein GI Number:
2507300
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P35250
User NOte:
Optimal dilutions for each application to be determined by the researcher.