POLR1B Antibody
| Code | Size | Price |
|---|
| PSI-56-464-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
DNA-directed RNA polymerase I subunit RPA2, RNA polymerase I subunit 2, DNA-directed RNA polymerase I 135 kDa polypeptide, RPA135, POLR1B
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
Eukaryotic RNA polymerase I (pol I) is responsible for the
transcription of ribosomal RNA (rRNA) genes and production of rRNA,
the primary component of ribosomes. Pol I is a multisubunit enzyme
composed of 6 to 14 polypeptides, depending on the species. Most of
the mass of the pol I complex derives from the 2 largest subunits,
Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and
Grummt, 1996 [PubMed 8921381]).
Background References:
- Wen, L., et al. Biochem. Biophys. Res. Commun. 367(4):846-851(2008)
- Wu, C., et al. Proteomics 7(11):1775-1785(2007)
- Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)
- Panova, T.B., et al. Mol. Cell. Biol. 26(16):5957-5968(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This POLR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the N-terminal region of human POLR1B.
NCBI Gene ID #:
84172
NCBI Official Name:
DNA-directed RNA polymerase I subunit RPA2
NCBI Official Symbol:
POLR1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
128 kDa
Protein Accession #:
Q9H9Y6
Protein GI Number:
92090637
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9H9Y6
User NOte:
Optimal dilutions for each application to be determined by the researcher.