EIF2A Antibody

ProSci
Product Code: PSI-63-985
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-985-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of EIF2A Antibody in Ramos cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human brain tissue reacted with EIF2A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of EIF2A Antibody in Ramos cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with EIF2A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Eukaryotic translation initiation factor 2A, eIF-2A, 65 kDa eukaryotic translation initiation factor 2A, Eukaryotic translation initiation factor 2A, N-terminally processed, EIF2A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).
Background References:
  • Willis, K.L., et al. Virology 394(1):73-81(2009)
  • Spurgeon, M.E., et al. J. Virol. 83(19):9970-9982(2009)
  • Lu, W., et al. J. Biol. Chem. 284(36):24281-24288(2009)
  • Yang, J., et al. Mol. Cell. Biol. 29(8):2243-2253(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EIF2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-465 amino acids from the Central region of human EIF2A.
NCBI Gene ID #:
83939
NCBI Official Name:
Eukaryotic translation initiation factor 2A
NCBI Official Symbol:
EIF2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
Q9BY44
Protein GI Number:
209572747
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9BY44
User NOte:
Optimal dilutions for each application to be determined by the researcher.