HOXA3 Antibody

ProSci
Product Code: PSI-64-170
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-170-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of HOXA3 Antibody in HL-60 cell line lysates (35ug/lane)
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HOXA3 Antibody IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of HOXA3 Antibody in HL-60 cell line lysates (35ug/lane)
HOXA3 Antibody IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Homeobox protein Hox-A3, Homeobox protein Hox-1E, HOXA3, HOX1E
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation.
Background References:
  • Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
  • Chung, N., et al. Mol. Biol. Rep. 36(2):227-235(2009)
  • Wissmuller, S., et al. Nucleic Acids Res. 34(6):1735-1744(2006)
  • Manzanares, M., et al. EMBO J. 21(3):365-376(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Zebrafish
Immunogen:
This HOXA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-440 amino acids from the C-terminal region of human HOXA3.
NCBI Gene ID #:
3200
NCBI Official Name:
Homeobox protein Hox-A3
NCBI Official Symbol:
HOXA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
O43365
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O43365
User NOte:
Optimal dilutions for each application to be determined by the researcher.