HSPA5 Antibody

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ProSci
Product Code: PSI-61-766
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-766-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A375,A2058,Hela cell line lysates (35ug/lane).
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HSPA5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in A375,A2058,Hela cell line lysates (35ug/lane).
HSPA5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.
Background References:
  • Zhao, C., et al. J. Med. Virol. 82(1):14-22(2010)
  • Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010)
  • Arnaudeau, S., et al. Proteomics 9(23):5316-5327(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat, Xenopus
Immunogen:
This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-568 amino acids from the C-terminal region of human HSPA5.
NCBI Gene ID #:
3309
NCBI Official Name:
78 kDa glucose-regulated protein
NCBI Official Symbol:
HSPA5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
72 kDa
Protein Accession #:
P11021
Protein GI Number:
14916999
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P11021
User NOte:
Optimal dilutions for each application to be determined by the researcher.