EIF3G Antibody
| Code | Size | Price |
|---|
| PSI-61-891-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Eukaryotic translation initiation factor 3 subunit G {ECO:0000255|HAMAP-Rule:MF_03006}, eIF3g {ECO:0000255|HAMAP-Rule:MF_03006}, Eukaryotic translation initiation factor 3 RNA-binding subunit {ECO:0000255|HAMAP-Rule:MF_03006}, eIF-3 RNA-binding subunit {ECO:0000255|HAMAP-Rule:MF_03006}, Eukaryotic translation initiation factor 3 subunit 4 {ECO:0000255|HAMAP-Rule:MF_03006}, eIF-3-delta {ECO:0000255|HAMAP-Rule:MF_03006}, eIF3 p42 {ECO:0000255|HAMAP-Rule:MF_03006}, eIF3 p44 {ECO:0000255|HAMAP-Rule:MF_03006}, EIF3G {ECO:0000255|HAMAP-Rule:MF_03006}
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Background:
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
Background References:
- Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
- Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)
- Damoc, E., et al. Mol. Cell Proteomics 6(7):1135-1146(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Zebrafish, Mouse, Rat
Immunogen:
This EIF3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the Central region of human EIF3G.
NCBI Gene ID #:
8666
NCBI Official Name:
Eukaryotic translation initiation factor 3 subunit G {ECO:0000255|HAMAP-Rule:MF_03006}
NCBI Official Symbol:
EIF3G
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
O75821
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O75821
User NOte:
Optimal dilutions for each application to be determined by the researcher.