PR38A Antibody

ProSci
Product Code: PSI-64-191
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-191-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis in MDA-MB231,CEM,HL-60 cell line lysates (35ug/lane).
3 / 4
4 / 4
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB231,CEM,HL-60 cell line lysates (35ug/lane).
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Pre-mRNA-splicing factor 38A, PRPF38A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background References:
  • Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Xenopus
Immunogen:
This PR38A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-312 amino acids from the C-terminal region of human PR38A.
NCBI Gene ID #:
84950
NCBI Official Name:
Pre-mRNA-splicing factor 38A
NCBI Official Symbol:
PRPF38A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
Q8NAV1
Protein GI Number:
74760086
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8NAV1
User NOte:
Optimal dilutions for each application to be determined by the researcher.