RUNX3 Antibody

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ProSci
Product Code: PSI-57-901
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-901-400ul400ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from HL-60, Jurkat cell line (from left to right), using RUNX3 Antibody at 1:1000 at each lane.
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RUNX3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysates from HL-60, Jurkat cell line (from left to right), using RUNX3 Antibody at 1:1000 at each lane.
RUNX3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, Core-binding factor subunit alpha-3, CBF-alpha-3, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEA2-alpha C, PEBP2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, RUNX3, AML2, CBFA3, PEBP2A3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Tsang, Y.H., et al. Oncogene 29(41):5643-5650(2010)
  • Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
  • Iwatani, K., et al. Biochem. Biophys. Res. Commun. 400(3):426-431(2010)
  • Kodach, L.L., et al. Carcinogenesis 31(9):1567-1575(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RUNX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human RUNX3.
NCBI Gene ID #:
864
NCBI Official Name:
Runt-related transcription factor 3
NCBI Official Symbol:
RUNX3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
Q13761
Protein GI Number:
17368453
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q13761
User NOte:
Optimal dilutions for each application to be determined by the researcher.