SIX5 Antibody

ProSci
Product Code: PSI-56-618
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-618-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
2 / 3
SIX5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
SIX5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Homeobox protein SIX5, DM locus-associated homeodomain protein, Sine oculis homeobox homolog 5, SIX5, DMAHP
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this gene are a cause of branchiootorenal syndrome type 2.
Background References:
  • Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
  • Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
  • Hoskins, B.E., et al. Am. J. Hum. Genet. 80(4):800-804(2007)
  • Sato, S., et al. Hum. Mol. Genet. 11(9):1045-1058(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This SIX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the Central region of human SIX5.
NCBI Gene ID #:
147912
NCBI Official Name:
Homeobox protein SIX5
NCBI Official Symbol:
SIX5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
Q8N196
Protein GI Number:
150421671
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8N196
User NOte:
Optimal dilutions for each application to be determined by the researcher.