RARS Antibody

ProSci
Product Code: PSI-55-620
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-620-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
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RARS Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in MCF-7 cell line lysates (35ug/lane).
RARS Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Arginine--tRNA ligase, cytoplasmic, Arginyl-tRNA synthetase, ArgRS, RARS
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq].
Background References:
  • Bottoni, A., et al. J. Cell. Physiol. 212(2):293-297(2007)
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
  • Ling, C., et al. J. Biol. Chem. 280(41):34755-34763(2005)
  • Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 605-634 amino acids from the C-terminal region of human RARS.
NCBI Gene ID #:
5917
NCBI Official Name:
Arginine--tRNA ligase, cytoplasmic
NCBI Official Symbol:
RARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
P54136
Protein GI Number:
20178331
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P54136
User NOte:
Optimal dilutions for each application to be determined by the researcher.