WDR52 Antibody

ProSci
Product Code: PSI-55-931
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-931-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).
2 / 4
WDR52 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 4
4 / 4
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in A549 cell line lysates (35ug/lane).
WDR52 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Cilia- and flagella-associated protein 44 {ECO:0000312|HGNC:HGNC:25631}, WD repeat-containing protein 52 {ECO:0000312|HGNC:HGNC:25631}, CFAP44 (HGNC:25631)
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The specific function of the protein remains unknown.
Background References:
  • Kimura, K., et al. Genome Res. 16(1):55-65(2006)
  • Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002)
  • Bonaldo, M.F., et al. Genome Res. 6(9):791-806(1996)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This WDR52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 768-797 amino acids from the C-terminal region of human WDR52.
NCBI Gene ID #:
55779
NCBI Official Name:
Cilia- and flagella-associated protein 44 {ECO:0000312|HGNC:HGNC:25631}
NCBI Official Symbol:
CFAP44
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
112 kDa
Protein Accession #:
Q96MT7
Protein GI Number:
74760982
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96MT7
User NOte:
Optimal dilutions for each application to be determined by the researcher.