EEF1B2 Antibody

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ProSci
Product Code: PSI-61-031
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-031-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Flow cytometric analysis of Hela cells using EEF1B2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Fluorescent image of Hela cells stained with EEF1B2 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Western blot analysis of lysates from Hela, K562 cell line (from left to right), using EEF1B2 Antibody at 1:1000 at each lane.
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Flow cytometric analysis of Hela cells using EEF1B2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Fluorescent image of Hela cells stained with EEF1B2 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from Hela, K562 cell line (from left to right), using EEF1B2 Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B, EF1B
Application Note:
For FACS starting dilution is: 1:25

For IF starting dilution is: 1:25

For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.
Background References:
  • Sanders J.,et al.Nucleic Acids Res. 19:4551-4551(1991).
  • von der Kammer H.,et al.Biochem. Biophys. Res. Commun. 177:312-317(1991).
  • Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
  • Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken
Immunogen:
This EEF1B2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 54-86 amino acids from the Central region of human EEF1B2.
NCBI Gene ID #:
1933
NCBI Official Name:
Elongation factor 1-beta
NCBI Official Symbol:
EEF1B2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
P24534
Protein GI Number:
119163
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P24534
User NOte:
Optimal dilutions for each application to be determined by the researcher.