NPM1 Antibody

Contact us
ProSci
Product Code: PSI-61-535
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-535-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Fluorescent image of A549 cell stained with NPM1 Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly.
2 / 4
Western blot analysis in Hela,Jurkat cell line lysates (35ug/lane).
3 / 4
4 / 4
NPM1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
❮❯

Fluorescent image of A549 cell stained with NPM1 Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly.
Western blot analysis in Hela,Jurkat cell line lysates (35ug/lane).
NPM1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, NPM1, NPM
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF. NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia.
Background References:
  • Vascotto,C., Mol. Cell. Biol. 29 (7), 1834-1854 (2009) Ma,W., Cancer Biomark(1), 51-58 (2009) Zhang,H., J. Biol. Chem. 279 (34), 35726-35734 (2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NPM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the C-terminal region of human NPM1.
NCBI Gene ID #:
4869
NCBI Official Name:
Nucleophosmin
NCBI Official Symbol:
NPM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
P06748
Protein GI Number:
114762
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P06748
User NOte:
Optimal dilutions for each application to be determined by the researcher.