DARS Antibody

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ProSci
Product Code: PSI-55-617
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-617-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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Western blot analysis of DARS using rabbit polyclonal DARS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.
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Confocal immunofluorescent analysis of DARS Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Western blot analysis of DARS using rabbit polyclonal DARS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.
Confocal immunofluorescent analysis of DARS Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Aspartate--tRNA ligase, cytoplasmic, Aspartyl-tRNA synthetase, AspRS, Cell proliferation-inducing gene 40 protein, DARS
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.
Background References:
  • Wu, C., et al. Proteomics 7(11):1775-1785(2007)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Tu, L.C., et al. Mol. Cell Proteomics 6(4):575-588(2007)
  • Ling, C., et al. J. Biol. Chem. 280(41):34755-34763(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This DARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the N-terminal region of human DARS.
NCBI Gene ID #:
1615
NCBI Official Name:
Aspartate--tRNA ligase, cytoplasmic
NCBI Official Symbol:
DARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
P14868
Protein GI Number:
20178330
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P14868
User NOte:
Optimal dilutions for each application to be determined by the researcher.