KIF11 Antibody

ProSci
Product Code: PSI-63-779
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-779-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Hamster
  • Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of KIF11 Antibody in CHO cell line lysates (35ug/lane)
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4 / 4
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of KIF11 Antibody in CHO cell line lysates (35ug/lane)
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Kinesin-like protein KIF11, Kinesin-like protein 1, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP-5, KIF11, EG5, KNSL1, TRIP5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
KIF11 belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
Background References:
  • Tcherniuk,S., et.al., Biochem. Pharmacol. 79 (6), 864-872 (2010)
  • Parke,C.L., et.al., J. Biol. Chem. 285 (8), 5859-5867 (2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KIF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the N-terminal region of human KIF11.
NCBI Gene ID #:
3832
NCBI Official Name:
Kinesin-like protein KIF11
NCBI Official Symbol:
KIF11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
119 kDa
Protein Accession #:
P52732
Protein GI Number:
116242604
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P52732
User NOte:
Optimal dilutions for each application to be determined by the researcher.