RPL27A Antibody
Code | Size | Price |
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PSI-55-518-400ul | 400ul | £626.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Hamster
- Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
60S ribosomal protein L27a, RPL27A
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Background:
Ribosomes, the organelles that catalyze protein synthesis,
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 60S subunit. The protein belongs
to the L15P family of ribosomal proteins. It is located in the
cytoplasm. Variable expression of this gene in colorectal cancers
compared to adjacent normal tissues has been observed, although no
correlation between the level of expression and the severity of the
disease has been found. As is typical for genes encoding ribosomal
proteins, multiple processed pseudogenes derived from this gene are
dispersed through the genome.
Background References:
- Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
- Mazumder, B., et al. Cell 115(2):187-198(2003)
- Andersen, J.S., et al. Curr. Biol. 12(1):1-11(2002)
- Bortoluzzi, S., et al. Bioinformatics 17(12):1152-1157(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Rat
Immunogen:
This RPL27A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-138 amino acids from the C-terminal region of human RPL27A.
NCBI Gene ID #:
6157
NCBI Official Name:
60S ribosomal protein L27a
NCBI Official Symbol:
RPL27A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
P46776
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P46776
User NOte:
Optimal dilutions for each application to be determined by the researcher.