OGG1 Antibody

ProSci
Product Code: PSI-62-546
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-546-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: NCCIT whole cell lysate Lane 6: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of OGG1 Antibody in Hela(lane 1), NIH-3T3(lane 2) cell line lysates (35ug/lane)

Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: NCCIT whole cell lysate Lane 6: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of OGG1 Antibody in Hela(lane 1), NIH-3T3(lane 2) cell line lysates (35ug/lane)

Further Information

Additional Names:
N-glycosylase/DNA lyase, 8-oxoguanine DNA glycosylase, 322-, DNA-(apurinic or apyrimidinic site) lyase, AP lyase, OGG1, MMH, MUTM, OGH1
Application Note:
For WB starting dilution is: 1:1000
Background:
OGG1 is the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage.
Background References:
  • Wozniak,K., et.al., J. Biomed. Biotechnol. 2009, 827562 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This OGG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-345 amino acids from the C-terminal region of human OGG1.
NCBI Gene ID #:
4968
NCBI Official Name:
N-glycosylase/DNA lyase
NCBI Official Symbol:
OGG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
O15527
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
O15527
User NOte:
Optimal dilutions for each application to be determined by the researcher.