DCBLD2 Antibody

ProSci
Product Code: PSI-57-298
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-298-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse NIH-3T3,Neuro-2a cell line and mouse stomach tissue and U251,A2058,A375,Sk-Br-3,T47D cell line lysates (35ug/lane).
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Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in mouse NIH-3T3,Neuro-2a cell line and mouse stomach tissue and U251,A2058,A375,Sk-Br-3,T47D cell line lysates (35ug/lane).
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Discoidin, CUB and LCCL domain-containing protein 2, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Endothelial and smooth muscle cell-derived neuropilin-like protein, DCBLD2, CLCP1, ESDN
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
DCBLD2, otherwise known as ESDN (endothelial and smooth muscle cell-derived neuropilin-like molecule) is a novel type-I transmembrane protein with the longest cleavable secretory signal sequence among eukaryotes. It is expressed in various tissues; particularly highly expressed in cultured vascular smooth muscle cells. DCBLD2 is considered to play a role in regulation of vascular cell growth and may have a wide variety of functions in other tissues including the nervous system, like neuropilins.
Background References:
  • Rose, J. Phd, et al. Mol. Med. (2010) In press :
  • Guo, X., et al. J. Biol. Chem. 284(43):29376-29382(2009)
  • Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009)
  • Kim, M., et al. Mol. Cancer Res. 6(2):222-230(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DCBLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 538-567 amino acids from the C-terminal region of human DCBLD2.
NCBI Gene ID #:
131566
NCBI Official Name:
Discoidin, CUB and LCCL domain-containing protein 2
NCBI Official Symbol:
DCBLD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
Q96PD2
Protein GI Number:
57013820
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
Q96PD2
User NOte:
Optimal dilutions for each application to be determined by the researcher.