JMJD2C Antibody

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ProSci
Product Code: PSI-55-851
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-851-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 4
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: K562 whole cell lysates Lane 3: LNCaP whole cell lysates Lane 4: mouse brain lysates Lysates/proteins at 20 ug per lane.
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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: K562 whole cell lysates Lane 3: LNCaP whole cell lysates Lane 4: mouse brain lysates Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Lysine-specific demethylase 4C, 11411-, Gene amplified in squamous cell carcinoma 1 protein, GASC-1 protein, JmjC domain-containing histone demethylation protein 3C, Jumonji domain-containing protein 2C, KDM4C, GASC1, JHDM3C, JMJD2C, KIAA0780
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background:
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with one JmjC domain, one JmjN domain, two PHD-type zinc fingers, and two Tudor domains. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form. Chromosomal aberrations and increased transcriptional expression of this gene are associated with esophageal squamous cell carcinoma. Alternative splicing results in multiple transcript variants.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Suikki, H.E., et al. Prostate 70(8):889-898(2010)
  • Kantojarvi, K., et al. Psychiatr. Genet. 20(3):102-108(2010)
  • Liu, G., et al. Oncogene 28(50):4491-4500(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This JMJD2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1023-1056 amino acids from the C-terminal region of human JMJD2C.
NCBI Gene ID #:
23081
NCBI Official Name:
Lysine-specific demethylase 4C
NCBI Official Symbol:
KDM4C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
120 kDa
Protein Accession #:
Q9H3R0
Protein GI Number:
97536525
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
Q9H3R0
User NOte:
Optimal dilutions for each application to be determined by the researcher.