HRASLS3 Antibody

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ProSci
Product Code: PSI-62-032
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-032-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the detected HRASLS3 protein (arrow).
2 / 2
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the detected HRASLS3 protein (arrow).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
HRAS-like suppressor 3, HRSL3, Adipose-specific phospholipase A2, AdPLA, Group XVI phospholipase A1/A2, H-rev 107 protein homolog, HRAS-like suppressor 1, HREV107-1, HREV107-3, Renal carcinoma antigen NY-REN-65, PLA2G16, HRASLS3, HREV107
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
PLA2G16 specifically catalyzes the release of fatty acids from phospholipids in adipose tissue. It also has a weak lysophospholipase activity (By similarity). Tumor suppressor that may be involved in interferon-dependent cell death.
Background References:
  • Uyama, T., et al. Biochim. Biophys. Acta 1791(12):1114-1124(2009)
  • Duncan, R.E., et al. J. Biol. Chem. 283(37):25428-25436(2008)
  • Nazarenko, I., et al. J. Cell. Sci. 120 (PT 8), 1393-1404 (2007)
  • Nazarenko, I., et al. Am. J. Pathol. 169(4):1427-1439(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HRASLS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-132 amino acids from the C-terminal region of human HRASLS3.
NCBI Gene ID #:
11145
NCBI Official Name:
HRAS-like suppressor 3
NCBI Official Symbol:
PLA2G16
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18 kDa
Protein Accession #:
P53816
Protein GI Number:
20141767
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer
Swissprot #:
P53816
User NOte:
Optimal dilutions for each application to be determined by the researcher.