DDX53 Antibody

ProSci
Product Code: PSI-62-946
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-946-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of DDX53 antibody in mouse kidney tissue lysates (35ug/lane)
2 / 2
Flow cytometric analysis of WiDr cells using DDX53 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of DDX53 antibody in mouse kidney tissue lysates (35ug/lane)
Flow cytometric analysis of WiDr cells using DDX53 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Probable ATP-dependent RNA helicase DDX53, Cancer-associated gene protein, Cancer/testis antigen 26, CT26, DEAD box protein 53, DEAD box protein CAGE, DDX53, CAGE
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background References:
  • Shim,H., Lee,H. Biotechnol. Lett. 28 (24), 2057-2063 (2006)
  • Cho,B., Lee,H. Biochem. Biophys. Res. Commun. 307 (1), 52-63 (2003)
  • Cho,B., Lim,Y. Biochem. Biophys. Res. Commun. 292 (3), 715-726 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DDX53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 597-626 amino acids from the C-terminal region of human DDX53.
NCBI Gene ID #:
168400
NCBI Official Name:
Probable ATP-dependent RNA helicase DDX53
NCBI Official Symbol:
DDX53
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
71 kDa
Protein Accession #:
Q86TM3
Protein GI Number:
143811384
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer
Swissprot #:
Q86TM3
User NOte:
Optimal dilutions for each application to be determined by the researcher.