RPL15 Antibody
Code | Size | Price |
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PSI-56-636-400ul | 400ul | £626.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
60S ribosomal protein L15, RPL15, EC45
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
Ribosomes, the organelles that catalyze protein synthesis,
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 60S subunit. The protein belongs
to the L15E family of ribosomal proteins. It is located in the
cytoplasm. This gene shares sequence similarity with the yeast
ribosomal protein YL10 gene. Although this gene has been referred
to as RPL10, its official symbol is RPL15. This gene has been shown
to be overexpressed in some esophageal tumors compared to normal
matched tissues. Transcript variants utilizing alternative polyA
signals exist. As is typical for genes encoding ribosomal proteins,
there are multiple processed pseudogenes of this gene dispersed
through the genome.
Background References:
- Wheeler, H.E., et al. PLoS Genet.(10), E1000685 (2009) :
- Wang, H., et al. BMC Cancer 6, 91 (2006) :
- Andersen, J.S., et al. Nature 433(7021):77-83(2005)
- Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Rat
Immunogen:
This RPL15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human RPL15.
NCBI Gene ID #:
6138
NCBI Official Name:
60S ribosomal protein L15
NCBI Official Symbol:
RPL15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
P61313
Protein GI Number:
47117726
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
P61313
User NOte:
Optimal dilutions for each application to be determined by the researcher.