Mouse Nek6 Antibody

ProSci
Product Code: PSI-57-356
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-356-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse liver tissue lysates (35ug/lane).
2 / 3
Western blot analysis of Nek6 using rabbit polyclonal Mouse Nek6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek6 gene.
3 / 3

Western blot analysis in mouse liver tissue lysates (35ug/lane).
Western blot analysis of Nek6 using rabbit polyclonal Mouse Nek6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek6 gene.

Further Information

Additional Names:
Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Nek6
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis (By similarity).
Background References:
  • Jeon, Y.J., et al. J. Biol. Chem. 285(36):28126-28133(2010)
  • Kaput, J., et al. Physiol. Genomics 18(3):316-324(2004)
  • Visel, A., et al. Nucleic Acids Res. 32 (DATABASE ISSUE), D552-D556 (2004) :
  • Hashimoto, Y., et al. Biochem. Biophys. Res. Commun. 293(2):753-758(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig, Rat
Immunogen:
This Mouse Nek6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-280 amino acids from the C-terminal region of mouse Nek6.
NCBI Gene ID #:
59126
NCBI Official Name:
Serine/threonine-protein kinase Nek6
NCBI Official Symbol:
Nek6
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
Q9ES70
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q9ES70
User NOte:
Optimal dilutions for each application to be determined by the researcher.