GAS7 Antibody

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ProSci
Product Code: PSI-62-379
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-379-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of GAS7 antibody in A2058 cell line lysates (35ug/lane)
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4 / 5
Formalin-fixed and paraffin-embedded human brain tissue reacted with GAS7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:1000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of GAS7 antibody in A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with GAS7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Growth arrest-specific protein 7, GAS-7, GAS7, KIAA0394
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development.
Background References:
  • Chao,C.C., J. Neurosci. Res. 81 (2), 153-162 (2005)
  • She,B.R., Exp. Cell Res. 273 (1), 34-44 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This GAS7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-465 amino acids from the C-terminal region of human GAS7.
NCBI Gene ID #:
8522
NCBI Official Name:
Growth arrest-specific protein 7
NCBI Official Symbol:
GAS7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
O60861
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle
Swissprot #:
O60861
User NOte:
Optimal dilutions for each application to be determined by the researcher.